The original paper is in English. Non-English content has been machine-translated and may contain typographical errors or mistranslations. ex. Some numerals are expressed as "XNUMX".
Copyrights notice
The original paper is in English. Non-English content has been machine-translated and may contain typographical errors or mistranslations. Copyrights notice
Dalam kerja ini, kami melaporkan di tapak pertumbuhan nanopartikel emas (AuNPs) untuk penambahbaikan sensor resonans plasmon permukaan penghantaran (T-SPR) untuk mengesan immunoglobulin manusia G (IgG). IgG manusia telah digerakkan pada monolayer pemasangan sendiri yang diaktifkan daripada 11-mercaptoundecanoic pada substrat parut bersalut emas. Sistem T-SPR juga digunakan untuk memantau pembinaan cip sensor serta pengikatan IgG dan anti-IgG yang digabungkan dengan AuNPs. Selepas penjerapan khusus dengan IgG, isyarat T-SPR dipertingkatkan lagi oleh di tapak pertumbuhan AuNPs terikat dengan anti-IgG. Menggunakan konjugasi AuNP dan di tapak pertumbuhan AuNPs terikat, sensitiviti imunosensor IgG telah dipertingkatkan dengan dua urutan magnitud berbanding dengan itu tanpa AuNPs konjugasi.
Theerasak JUAGWON
Niigata University,Mahidol University
Chutiparn LERTVACHIRAPAIBOON
Niigata University
Kazunari SHINBO
Niigata University
Keizo KATO
Niigata University
Toemsak SRIKHIRIN
Mahidol University
Tanakorn OSOTCHAN
Mahidol University
Akira BABA
Niigata University
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Salinan
Theerasak JUAGWON, Chutiparn LERTVACHIRAPAIBOON, Kazunari SHINBO, Keizo KATO, Toemsak SRIKHIRIN, Tanakorn OSOTCHAN, Akira BABA, "Detection of Human Immunoglobulin G by Transmission Surface Plasmon Resonance Using the In Situ Gold Nanoparticle Growth Method" in IEICE TRANSACTIONS on Electronics,
vol. E102-C, no. 2, pp. 125-131, February 2019, doi: 10.1587/transele.2018OMP0004.
Abstract: In this work, we report the in situ growth of gold nanoparticles (AuNPs) for the improvement of a transmission surface plasmon resonance (T-SPR) sensor to detect human immunoglobulin G (IgG). Human IgG was immobilized on an activated self-assembled monolayer of 11-mercaptoundecanoic on a gold-coated grating substrate. The T-SPR system was also used to monitor the construction of sensor chips as well as the binding of IgG and anti-IgG conjugated with AuNPs. After specific adsorption with IgG, the T-SPR signal was further enhanced by the in situ growth of AuNPs bound with anti-IgG. Using AuNP conjugation and in situ growth of bound AuNPs, the sensitivity of the IgG immunosensor was improved by two orders of magnitude compared with that without conjugated AuNPs.
URL: https://global.ieice.org/en_transactions/electronics/10.1587/transele.2018OMP0004/_p
Salinan
@ARTICLE{e102-c_2_125,
author={Theerasak JUAGWON, Chutiparn LERTVACHIRAPAIBOON, Kazunari SHINBO, Keizo KATO, Toemsak SRIKHIRIN, Tanakorn OSOTCHAN, Akira BABA, },
journal={IEICE TRANSACTIONS on Electronics},
title={Detection of Human Immunoglobulin G by Transmission Surface Plasmon Resonance Using the In Situ Gold Nanoparticle Growth Method},
year={2019},
volume={E102-C},
number={2},
pages={125-131},
abstract={In this work, we report the in situ growth of gold nanoparticles (AuNPs) for the improvement of a transmission surface plasmon resonance (T-SPR) sensor to detect human immunoglobulin G (IgG). Human IgG was immobilized on an activated self-assembled monolayer of 11-mercaptoundecanoic on a gold-coated grating substrate. The T-SPR system was also used to monitor the construction of sensor chips as well as the binding of IgG and anti-IgG conjugated with AuNPs. After specific adsorption with IgG, the T-SPR signal was further enhanced by the in situ growth of AuNPs bound with anti-IgG. Using AuNP conjugation and in situ growth of bound AuNPs, the sensitivity of the IgG immunosensor was improved by two orders of magnitude compared with that without conjugated AuNPs.},
keywords={},
doi={10.1587/transele.2018OMP0004},
ISSN={1745-1353},
month={February},}
Salinan
TY - JOUR
TI - Detection of Human Immunoglobulin G by Transmission Surface Plasmon Resonance Using the In Situ Gold Nanoparticle Growth Method
T2 - IEICE TRANSACTIONS on Electronics
SP - 125
EP - 131
AU - Theerasak JUAGWON
AU - Chutiparn LERTVACHIRAPAIBOON
AU - Kazunari SHINBO
AU - Keizo KATO
AU - Toemsak SRIKHIRIN
AU - Tanakorn OSOTCHAN
AU - Akira BABA
PY - 2019
DO - 10.1587/transele.2018OMP0004
JO - IEICE TRANSACTIONS on Electronics
SN - 1745-1353
VL - E102-C
IS - 2
JA - IEICE TRANSACTIONS on Electronics
Y1 - February 2019
AB - In this work, we report the in situ growth of gold nanoparticles (AuNPs) for the improvement of a transmission surface plasmon resonance (T-SPR) sensor to detect human immunoglobulin G (IgG). Human IgG was immobilized on an activated self-assembled monolayer of 11-mercaptoundecanoic on a gold-coated grating substrate. The T-SPR system was also used to monitor the construction of sensor chips as well as the binding of IgG and anti-IgG conjugated with AuNPs. After specific adsorption with IgG, the T-SPR signal was further enhanced by the in situ growth of AuNPs bound with anti-IgG. Using AuNP conjugation and in situ growth of bound AuNPs, the sensitivity of the IgG immunosensor was improved by two orders of magnitude compared with that without conjugated AuNPs.
ER -